Nadine Henderson

Research Support Specialist
nadine.henderson@stonybrook.edu

Laboratory of Dr. David Thanassi, 2002 - present

B.S., 1991, University of California, Davis
M.S., 1993, Indiana University

Scottish Crop Research Institute, 1994 - 1996
University of Dundee (Scotland), Department of Anatomy and Physiology, 1996 - 1997
University of Dundee (Scotland), Department of Molecular and Cellular Pathology, 1997 - 2000
Stony Brook University, Department of Pathology, 2000 - 2002

I study the structure and mechanism of chaperone usher pathways that assemble adhesive pili that are required for E coli pathogenesis.

Select Publications:
Bisgrove SR, Simonich MT, Smith NM, Sattler A, and RW Innes. 1994. A disease resistance gene in Arabidopsis with specificity for two different pathogen avirulence genes. Plant Cell. 6:927-933.

Henderson NS, Nijtmans LG, Lindsay JG, Lamantea E, Zeviani M, and IJ Holt. 2000. Separation of intact pyruvate dehydrogenase complex using blue native agarose gel electrophoresis. Electrophoresis. 21:2925-2931.

Nijtmans LG, Henderson NS, Attardi G, and IJ Holt. 2001. Impaired ATP synthase assembly associated with a mutation in the human ATP synthase subunit 6 gene. J. Biol. Chem. 276:6755-6762.

Nijtmans LG, Henderson NS and IJ Holt. 2002. Blue Native electrophoresis to study mitochondrial and other protein complexes. Methods. 26:327-334.

Henderson NS, So SS, Martin C, Kulkarni R, and DG Thanassi. 2004. Topology of the outer membrane usher PapC determined by site-directed fluorescence labeling.J. Biol. Chem. 279:53747-53754.

Remaut H, Tang C, Henderson NS, Pinkner JS, Wang T, Hultgren SJ, Thanassi DG, Waksman G, and H Li. 2008. Fiber formation across the bacterial outer membrane by the chaperone/usher pathway. Cell. 133:640-652.

Mapingire OS, Henderson NS, Duret G, Thanassi DG, and AH Delcour. 2009. Modulating effects of the plug, helix, and N- and C-terminal domains on channel properties of the PapC usher. J. Biol. Chem. 284:36324-36333.

Henderson NS, Ng TW, Talukder I, and DG Thanassi. 2011. Function of the usher N-terminus in catalysing pilus assembly. Mol. Microbiol. 79:954-967.

Phan G, Remaut H, Wang T, Allen WJ, Pirker KF, Lebedev A, Henderson NS, Geibel S, Volkan E, Yan J, Kunze MB, Pinkner JS, Ford B, Kay CW, Li H, Hultgren SJ, Thanassi DG, and G Waksman. 2011. Crystal structure of the FimD usher bound to its cognate FimC-FimH substrate. Nature. 474:49-53.

Volkan E, Ford BA, Pinkner JS, Dodson KW, Henderson NS, Thanassi DG, Waksman G, and SJ Hultgren. 2012. Domain activities of PapC usher reveal the mechanism of action of an Escherichia coli molecular machine. Proc. Natl. Acad. Sci. U. S. A. 109:9563-9568.

Henderson NS and DG Thanassi. 2013. Purification of the outer membrane usher protein and periplasmic chaperone-subunit complexes from the P and type 1 pilus systems. Methods Mol. Biol. 966:37-52.

Volkan E, Kalas V, Pinkner JS, Dodson KW, Henderson NS, Pham T, Waksman G, Delcour AH, Thanassi DG, and SJ Hultgren. 2013. Molecular basis of usher pore gating in Escherichia coli pilus biogenesis. Proc. Natl. Acad. Sci. U. S. A. 110:20741-20746.

Farabella I, Pham T, Henderson NS, Geibel S, Phan G, Thanassi DG, Delcour AH, Waksman G, and M Topf. 2014. Allosteric signaling in the outer membrane translocation domain of PapC usher. Elife. 3. doi: 10.7554/eLife.03532.

Werneburg GT, Henderson NS, Portnoy EB, Sarowar S, Hultgren SJ, Li H, and DG Thanassi. 2015. The pilus usher controls protein interactions via domain masking and is functional as an oligomer. Nat. Struct. Mol. Biol. 22:540-546.

Pham, T, Henderson, NS, Werneburg, DT, Thanassi, DG, and AH Delcour. 2015. Electrostatic networks control plug stabilization in the PapC usher. Mol. Membr. Biol. 32(5-8):198-207.

Pham, T, Werneburg, GT, Henderson, NS, Thanassi, DG, and AH Delcour. 2016. Effect of chaperone-adhesin complex on plug release by the PapC usher. FEBS Lett. 590 (14): 2172-9.

Du, M, Yuan, Z, Yu, H, Henderson, NS, Sarowar, S, Zhao, G, Werneburg, GT, Thanassi, DG, and H Li. 2018. Handover mechanism of the growing pilus by the bacterial outer-membrane usher FimD. Nature. 562(7727):444-447.

Psonis, JJ, Chahales, P, Henderson, NS, Rigel, NW, Hoffman, PS, and DG Thanassi. 2019. The small molecule nitazoxanide selectively disrupts BAM-mediated folding of the outer membrane usher protein. J Biol. Chem. 294(39):14357-14369.

Du, M, Yuan, Z, Werneburg, GT, Henderson, NS, Chauhan, H, Kovach, A, Zhao, G, Johl, J, Li, H, and DG Thanassi. 2021. Processive dynamics of the usher assembly platform during uropathogenic Escherichia coli P pilus biogenesis. Nat. Commun. 12(1):5207.